If evaporation occurs at the edges, tighten lid an additional 1/4 turn of the thumbwheel. Continue to turn the thumbwheel about 1/2–3/4 turn past the point of first contact (1/4 of the thumbwheel circumference is visible, so two passes of the visible portion equals 1/2 turn).Ĥ. 5ml tubes, microarrays, biochips or flat bottom vessels, FAM, SYBR Green, HEX. Heated lids, turn the thumbwheel until the lid contacts the plate - when an increase in resistance is first felt. A simple PCR machine like Bio-Rad T100 thermal cycler has a list price of. When using MJ thermal cyclers with adjustable Apply pressure with fingers or soft rubber roller to complete contact with each well. Remove white liner and place adhesive surface over wells. Minimum recommended PCR sample volumes are 25 µl in 96-well PCR plates and 5 µl in 384-well plates.ĭirections for Use 1. It is thin enough to pierce with a pipet tip to recover samples from individual wells. This aluminized foil serves as an exceptional barrier against evaporation from –70☌ to 105☌. Reusable sealing pad adheres to the heated lid.Smooth release - no aerosol generation.Plate storage or transport before or after cycling.Clear adhesive seal for thermal cycling or fluorescent assays.Peel-away release liner for pristine sealing surface.Smooth application and release - no aerosol generation.Choice of dome caps or ultraclear, flat optical caps Find the best PCR plates 48-well, 96-well and 384-well plates including low and high profile, unskirted, semi-skirted, or skirted optional colors and barcodes.Tight sealing for thermal cycling, storage, or transport.Foil can be pierced for sample recovery.
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